Arnold Group at the University of Wisconsin Milwaukee-Research: Organic Chemistry and Parallel Synthesis

Arnold Group at the University of Wisconsin Milwaukee-Research: Organic Chemistry and Parallel Synthesis

Dr. Premchendar Nandhikonda

Organic Chemistry and Parallel Synthesis

Our approach to synthesis is driven by the identification of relevant compounds using high throughput screening of computational methods.

Most reaction conditions are optimized using traditional methods of organic synthesis.

The synthesis of compound analogs to determine Structure-Activity Relationships are carried out using a carousel, the starfish or multi-plate wells (96)
Arnold Group at the University of Wisconsin Milwaukee-Research: Structure-Activity Relationships

Arnold Group at the University of Wisconsin Milwaukee-Research: SP1 (Biotage) for Purification

Kelly Teske
Serial automated purification is carried out using a SP1 (Biotage) . We apply the purification method for starting materials of intermediates usually between 0.01g-10g.
Libraries with many analogs are purified using an automated reverse phase HPLC system (Shimadzu). Pure factions are pooled and evaporated using a personal evaporated EZ2 (Genevac)

Revathi Kodali

Phani Babu
Determination of compound purity and identity is carried out using LCMS (Shimadzu 2020). This method also allows us to monitor product formation of chemical reactions and identification of pure HPLC fractions.

 
University of Wisconsin-Milwaukee Department of Chemistry and Biochemistry-Arnold Group Chemistry Building 3210 Cramer Street Milwaukee, WI 53211-3029